It seems you’re interested in protocols and laboratory concepts, particularly in a scientific or research context. Here are some key notions and concepts related to laboratory work, along with some general guidelines for creating and following protocols:


  1. Large scale Ion Exchange Column Purification  (3-100 liters) 
  2. Small scale Ion Exchange column purification (0.25-3 liters)
  3. Integration Host Factor purification protocol
  4. Recombinase Xis purification protocol
  5. Western Blot protocol
  6. Integration Recombination and bandshift buffers
  7. Electrocompetent cells
  8. Buffers of electrocompetent cells
  9. Polyethylene Glycol Electrofusion¬†“PFGE”
  10. Minimum Inhibitory Concentration¬†“MIC”
  11. Electric Microscopy
  12. Small-scale electroporation

Laboratory Notions and Concepts:

  1. Antibiotic
  2. Dye for DNA
  3. Alkaline lysis plasmid miniprep
  4. B-Galactosidase assay – Microtiter
  5. Genomic DNA
  6. Chemically-Competent Cell
  7. Electrocompetent cells
  8. Removing markers flanked by FRT sites
  9. P1 Transduction Escherichia coli
  10. Sam’s Red Swap Guide
  11. Arbitrary PCR