Lab Protocols

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  • Large scale Int purification (3-100 liters) PDF

  • Small scale Int purification (0.25-3 liters) PDF

  • IHF purification protocol PDF

  • Xis purification protocol PDF

  • Western protocol PDF

  • Int recombination and bandshift buffers PDF

  • Making electrocompetent cells PDF

  • Buffers PDF

  • PFGE PDF

  • MIC PDF

  • Microscopy PDF

  • TUNEL DNA Damage Detection Assay (updated) DOC

  • 96 Well Template (updated) XLS

  • Patching Grid (updated) PPT

  • Small scale electroporation PDF

  • Viability Experiment PDF

  • Antibiotic Information (updated) DOC

  • 6X Loading Dye for DNA DOC

  • Alkaline lysis plasmid miniprep DOC

  • B-Galactosidase assay - Microtiter plate format DOC

  • Genomic DNA preparation (CTAB method) DOC

  • Chemically-Competent Cell Recipe DOC

  • EBU plates DOC

  • Electrocompetent cells DOC

  • Protocol for removing markers flanked by FRT sites DOC

  • NCE Medium DOC

  • P1 Transduction (E. coli) DOC

  • DNase-free RNase DOC

  • Sam's Red Swap Guide DOC

  • Arbitrary PCR DOC

  • 4X SDS-PAGE Sample Loading Buffer DOC

  • Pfx50 Polymerase Formula XLS

  • Taq Formula XLS

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